Nests & setups

Test Tube Setup

Definition

A simple founding nest made from a test tube with water at the bottom, held back by a cotton plug, giving a queen and her young colony a small, dark and humid chamber.

Also known astest tube setupstest tube nest
Finished test tube setup with a water reservoir behind a cotton plug and a second cotton plug closing the entrance

The test tube setup is the most popular way to start an ant colony from a single queen. It's nothing more than a test tube about half filled with water, with a cotton ball pushed down to hold the water back. The queen lives in the dry part, drinks from the damp cotton and enjoys stable humidity, while a second cotton plug closes the entrance.

It works so well because it copies the founding chamber a queen digs in the wild: a tiny, quiet, humid space with barely enough room for her and her first brood. Queens hate large spaces, and a small chamber makes them feel safe.

When I catch a new Lasius niger or Messor barbarus queen, she goes straight into a test tube. It's cheap, it's easy to make, and you can check on the colony without opening anything. A young colony can happily live in one until the tube starts to fill up with workers.

In practice

  • Fill about half the tube with clean water, then push the cotton in quickly so no air bubbles get trapped against the water.
  • Wrap the tube in tinfoil or dark paper and keep it somewhere quiet, without vibrations or sudden light changes.
  • Don't move the colony to a big nest too soon. A test tube is enough until it gets crowded.
  • When the water runs low or mold appears, set up a fresh tube next to the old one and let the ants move on their own.

Related terms

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